KMID : 0352720080320040300
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Journal of Ginseng Research 2008 Volume.32 No. 4 p.300 ~ p.304
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Isolation of Cysteine Proteinase Gene (PgCysP1) from Panax ginseng and Response of This Gene to Abiotic Stresses
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Jung Dae-Young
Shim Ju-Sun Lee Jung-Hye In Jun-Gyo Lee Bum-Soo Yang Deok-Chun Kim Yu-Jin
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Abstract
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Cysteine proteinases play an essential role in plant growth and development but also in senescence and programmed cell death. They participate in both anabolic and catabolic processes. In addition, they are involved in signalling pathways and in the response to biotic and abiotic stresses. A cDNA clone encoding cysteine proteinase (CP) gene, designated PgCysP1, was isolated from Panax ginseng C. A. Meyer. Reverse transcriptase (RT)-PCR results showed that PgCysP1 expressed at different level in P. ginseng hairy root. Different stresses such as biotic as well as abiotic stresses triggered a significant induction of PgCysP1. The positive responses of PgCysP1 to the various stimuli suggested that PgCysP1 may help to protect the plant against reactive environmental stresses.
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KEYWORD
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Panax ginseng, cysteine proteinase(CP), abiotic stress, RT-PCR
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